rabbit anti itgb3 polyclonal antibody  (Cell Signaling Technology Inc)

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Enhancing endometrial receptivity: the roles of human chorionic gonadotropin in autophagy and apoptosis regulation in endometrial stromal cells

doi: 10.1186/s12958-024-01205-x

Figure Lengend Snippet: Endometrial receptivity is down-regulated in RIF patients. A The mRNA expression levels of HOXA10, ITGB3 and LHCGR in the endometrium of normal controls ( n = 12) and RIF patients ( n = 10). B WB was used to detect the protein levels of HOXA10, ITGB3 and LHCGR in endometrium of normal control ( n = 12) and RIF patients ( n = 10). Analyzing gels and western blots with ImageJ. Error bars, mean ± SD. * P < 0.05, ** P < 0.01. C Representative IHC images of LHCGR localization in endometrial tissue from normal control ( n = 12) and RIF patients ( n = 10) in secretory phase. Scale, 100 μm and 50 μm, analyzing relative OD with ImageJ. Error bars, mean ± SD. ** P < 0.01

Article Snippet: These membranes were saturated with blocking buffer for 1 h. Following this, membranes were incubated with rabbit polyclonal antiLHCGR (1:1,000 dilution; Proteintech), rabbit polyclonal anti-HOXA10 (1:1,000 dilution; Proteintech), rabbit polyclonal anti-ITGB3 antibodies (1:2,000 dilution; Proteintech), rabbit polyclonal anti-LIF antibodies (1:1,000 dilution; Proteintech) and mouse monoclonal anti-MECA-79 antibodies (1:500 dilution; Santa Cruz) at 4 °C.

Techniques: Expressing, Western Blot

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Enhancing endometrial receptivity: the roles of human chorionic gonadotropin in autophagy and apoptosis regulation in endometrial stromal cells

doi: 10.1186/s12958-024-01205-x

Figure Lengend Snippet: The expression levels of HOXA10, ITGB3, FOXO1, LIF and L-selectin ligand (MECA-79) in uterine ESCs after hCG intervention. A The mRNA expression levels of HOXA10, ITGB3, FOXO1 and LIF in ESCs were stimulated with hCG for 72 h. B The protein expression levels of HOXA10, ITGB3, FOXO1, LIF and L-selectin ligand (MECA-79) in ESCs were stimulated by hCG for 72 h. The data were shown as mean ± SD ( n = 3): * P < 0.05, ** P < 0.01, *** P < 0.001. C IF Representative images of ITGB3, LIF and L-selectin ligand (MECA-79) in ESCs treated with 0.1IU/mL hCG for 72 h. Scale: 100 μm

Article Snippet: These membranes were saturated with blocking buffer for 1 h. Following this, membranes were incubated with rabbit polyclonal antiLHCGR (1:1,000 dilution; Proteintech), rabbit polyclonal anti-HOXA10 (1:1,000 dilution; Proteintech), rabbit polyclonal anti-ITGB3 antibodies (1:2,000 dilution; Proteintech), rabbit polyclonal anti-LIF antibodies (1:1,000 dilution; Proteintech) and mouse monoclonal anti-MECA-79 antibodies (1:500 dilution; Santa Cruz) at 4 °C.

Techniques: Expressing

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Enhancing endometrial receptivity: the roles of human chorionic gonadotropin in autophagy and apoptosis regulation in endometrial stromal cells

doi: 10.1186/s12958-024-01205-x

Figure Lengend Snippet: The expression levels of LHCGR, FOXO1, ITGB3 ,HOXA10, LIF and L-selectin ligand in ESCs after the knockdown of LHCGR. A The mRNA expression levels of LCGR, HOXA10, ITGB3, FOXO1 and LIF in ESCs after the knockdown of LHCGR. B The protein expression levels of LHCGR in ESCs were stimulated by hCG and knockdown of LHCGR. C The protein expression levels of FOXO1, ITGB3, HOXA10, LIF and L-selectin ligand in ESCs after the knockdown of LHCGR. The data were shown as mean ± SD ( n = 3): * P < 0.05, ** P < 0.01

Article Snippet: These membranes were saturated with blocking buffer for 1 h. Following this, membranes were incubated with rabbit polyclonal antiLHCGR (1:1,000 dilution; Proteintech), rabbit polyclonal anti-HOXA10 (1:1,000 dilution; Proteintech), rabbit polyclonal anti-ITGB3 antibodies (1:2,000 dilution; Proteintech), rabbit polyclonal anti-LIF antibodies (1:1,000 dilution; Proteintech) and mouse monoclonal anti-MECA-79 antibodies (1:500 dilution; Santa Cruz) at 4 °C.

Techniques: Expressing

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Enhancing endometrial receptivity: the roles of human chorionic gonadotropin in autophagy and apoptosis regulation in endometrial stromal cells

doi: 10.1186/s12958-024-01205-x

Figure Lengend Snippet:

Article Snippet: These membranes were saturated with blocking buffer for 1 h. Following this, membranes were incubated with rabbit polyclonal antiLHCGR (1:1,000 dilution; Proteintech), rabbit polyclonal anti-HOXA10 (1:1,000 dilution; Proteintech), rabbit polyclonal anti-ITGB3 antibodies (1:2,000 dilution; Proteintech), rabbit polyclonal anti-LIF antibodies (1:1,000 dilution; Proteintech) and mouse monoclonal anti-MECA-79 antibodies (1:500 dilution; Santa Cruz) at 4 °C.

Techniques:

Journal: Molecular Medicine Reports

Article Title: Itgb3-integrin-deficient mice may not be a sufficient model for patients with Glanzmann thrombasthenia

doi: 10.3892/mmr.2021.12088

Figure Lengend Snippet: Fibrinogen binding and aggregation of Itgb3 −/− platelets. (A) Fibrinogen binding of washed platelets from three types of mice (Itgb3 +/+ , Itgb3 +/− and Itgb3 −/− mice) under stimulation by agonists, including ADP, AF, or Mn 2+ , with or without the inhibitor RGDS. (B) Whole blood aggregation of Itgb3 +/+ and Itgb3 −/− mice under stimulation of 2 µg/ml thrombin, 6 µg/ml collagen or 12 µg/ml collagen. Calcein AM-labeled whole blood from Itgb3 +/+ mice and Itgb3 −/− mice was perfused over a collagen-coated surface at shear rates of 1,500 s −1 and images were captured under (C) bright-field at the indicated time points and (D) fluorescence microscope at 4.5 min. Arrows indicated the adhered platelets. ADP, adenosine diphosphate; AF, Ala-Tyr-Pro-Gly-Lys-Phe (AYPGKF); Itgb3, integrin β3; RGDS, Arg-Gly-Asp-Ser.

Article Snippet: Rabbit anti-Itgb3 polyclonal antibody (cat. no. 4702) for western blotting was purchased from Cell Signaling Technology, Inc. Rabbit anti-β-actin polyclonal antibody (cat. no. 4968) for western blot was purchased from Cell Signaling Technology, Inc. HRP-conjugated goat anti-rabbit IgG secondary antibodies (cat. no. 5196-2504) was purchased from Bio-Rad, Laboratories, Inc. RIPA buffer (cat. no. 89900) and protease inhibitor mixture (cat. no. 87786) were purchased from Thermo Fisher Scientific, Inc.

Techniques: Binding Assay, Labeling, Shear, Fluorescence, Microscopy

Journal: Molecular Medicine Reports

Article Title: Itgb3-integrin-deficient mice may not be a sufficient model for patients with Glanzmann thrombasthenia

doi: 10.3892/mmr.2021.12088

Figure Lengend Snippet: Adhesion and spreading of Itgb3 −/− platelets. (A) Adhesion of washed Itgb3 +/+ and Itgb3 −/− platelets on the surfaces coated with Col, Fib, vWF or blank control. (B) Quantification of adhered platelets in (A). (C) Spreading of washed Itgb3 +/+ and Itgb3 −/− platelets on immobilized fibrinogen or control. TRITC-conjugated phalloidin was used to label actin (red). (D) Area coverage of spreading of washed Itgb3 +/+ and Itgb3 −/− platelets on immobilized fibrinogen in (C). *P<0.05 vs. Itgb3 +/+ . Col, collagen; Fib, fibrinogen; Itgb3, integrin β3; vWF, von Willebrand Factor.

Article Snippet: Rabbit anti-Itgb3 polyclonal antibody (cat. no. 4702) for western blotting was purchased from Cell Signaling Technology, Inc. Rabbit anti-β-actin polyclonal antibody (cat. no. 4968) for western blot was purchased from Cell Signaling Technology, Inc. HRP-conjugated goat anti-rabbit IgG secondary antibodies (cat. no. 5196-2504) was purchased from Bio-Rad, Laboratories, Inc. RIPA buffer (cat. no. 89900) and protease inhibitor mixture (cat. no. 87786) were purchased from Thermo Fisher Scientific, Inc.

Techniques: Control

Journal: Molecular Medicine Reports

Article Title: Itgb3-integrin-deficient mice may not be a sufficient model for patients with Glanzmann thrombasthenia

doi: 10.3892/mmr.2021.12088

Figure Lengend Snippet: Bleeding time and blood smear of Itgb3 −/− mice. (A) Bleeding of Itgb3 +/+ and Itgb3 −/− mice 8 min after tail cutting. Note the blood stains on the bottom of cage in Itgb3 −/− mouse group. (B) Bleeding time of different types of mice, *P<0.05 vs. Itgb3 +/+ mice; ✩ P<0.05 vs. Itgb3 +/− mice. (C) Subcutaneous hemorrhage in a Itgb3 −/− newborn pup. Arrow indicates the site of bleeding). (D) Wright staining of peripheral blood smear of Itgb3 −/− mice and Itgb3 +/+ mice. Note the microcytic hypochromic erythrocytes from Itgb3 −/− mice.

Article Snippet: Rabbit anti-Itgb3 polyclonal antibody (cat. no. 4702) for western blotting was purchased from Cell Signaling Technology, Inc. Rabbit anti-β-actin polyclonal antibody (cat. no. 4968) for western blot was purchased from Cell Signaling Technology, Inc. HRP-conjugated goat anti-rabbit IgG secondary antibodies (cat. no. 5196-2504) was purchased from Bio-Rad, Laboratories, Inc. RIPA buffer (cat. no. 89900) and protease inhibitor mixture (cat. no. 87786) were purchased from Thermo Fisher Scientific, Inc.

Techniques: Wright Stain

Journal: Molecular Medicine Reports

Article Title: Itgb3-integrin-deficient mice may not be a sufficient model for patients with Glanzmann thrombasthenia

doi: 10.3892/mmr.2021.12088

Figure Lengend Snippet: Whole blood count of different groups of mice.

Article Snippet: Rabbit anti-Itgb3 polyclonal antibody (cat. no. 4702) for western blotting was purchased from Cell Signaling Technology, Inc. Rabbit anti-β-actin polyclonal antibody (cat. no. 4968) for western blot was purchased from Cell Signaling Technology, Inc. HRP-conjugated goat anti-rabbit IgG secondary antibodies (cat. no. 5196-2504) was purchased from Bio-Rad, Laboratories, Inc. RIPA buffer (cat. no. 89900) and protease inhibitor mixture (cat. no. 87786) were purchased from Thermo Fisher Scientific, Inc.

Techniques:

Journal: Molecular Medicine Reports

Article Title: Itgb3-integrin-deficient mice may not be a sufficient model for patients with Glanzmann thrombasthenia

doi: 10.3892/mmr.2021.12088

Figure Lengend Snippet: Bone marrow and spleen of Itgb3 −/− mice. (A) Clustered erythroblasts are shown in bone marrow smear (Wright staining) from Itgb3 −/− mice (arrows). Magnification, ×1,000. (B) The number of erythroblasts increased in Itgb3 −/− compared with those in Itgb3 +/+ mouse bone marrow. Average number of erythroblasts in every 5 fields. n=3; *P<0.05 vs. Itgb3 +/+ mice. (C) Prussian blue staining shows decreased iron granules (arrows) in Itgb3 −/− bone marrow vs. Itgb3 +/+ bone marrow. Magnification, ×1,000. (D) Itgb3 −/− mice exhibited larger spleens compared with Itgb3 +/+ mice. Itgb3, integrin β3.

Article Snippet: Rabbit anti-Itgb3 polyclonal antibody (cat. no. 4702) for western blotting was purchased from Cell Signaling Technology, Inc. Rabbit anti-β-actin polyclonal antibody (cat. no. 4968) for western blot was purchased from Cell Signaling Technology, Inc. HRP-conjugated goat anti-rabbit IgG secondary antibodies (cat. no. 5196-2504) was purchased from Bio-Rad, Laboratories, Inc. RIPA buffer (cat. no. 89900) and protease inhibitor mixture (cat. no. 87786) were purchased from Thermo Fisher Scientific, Inc.

Techniques: Wright Stain, Staining

Journal: Molecular Medicine Reports

Article Title: Itgb3-integrin-deficient mice may not be a sufficient model for patients with Glanzmann thrombasthenia

doi: 10.3892/mmr.2021.12088

Figure Lengend Snippet: Spleen extramedullary hematopoiesis and iron deficiency of Itgb3 −/− mice. (A) Relative ratio of each organ to whole body weight in different genotype mice. *P<0.01 vs. Itgb3 +/+ mice; ✩ P<0.01 vs. Itgb3 +/− mice. (B) Relative ratio of spleen to whole body weight in Itgb3 −/− , Itgb3 +/− , Itgb3 +/+ and CHM mice. *P<0.01 vs. Itgb3 +/+ mice; ✩ P<0.01 vs. Itgb3 +/− mice. Immunohistochemical study of spleen biopsy from Itgb3 +/+ , Itgb3 −/− mice. Anti-CD3 antibody was used to label T lymphocytes, anti-CD19 antibody to label B lymphocytes and anti-CD71 antibody to label erythrocytes. Magnification (C) ×100 and (D) ×600. (E) Concentration of plasma ferritin in Itgb3 −/− mice. (F) OD value of fecal occult blood in Itgb3 −/− mice using ELISA. CHM, chronic hemorrhagic model; H&E, hematoxylin and eosin; Itgb3, integrin β3; OD, optical density; ns, not significant.

Article Snippet: Rabbit anti-Itgb3 polyclonal antibody (cat. no. 4702) for western blotting was purchased from Cell Signaling Technology, Inc. Rabbit anti-β-actin polyclonal antibody (cat. no. 4968) for western blot was purchased from Cell Signaling Technology, Inc. HRP-conjugated goat anti-rabbit IgG secondary antibodies (cat. no. 5196-2504) was purchased from Bio-Rad, Laboratories, Inc. RIPA buffer (cat. no. 89900) and protease inhibitor mixture (cat. no. 87786) were purchased from Thermo Fisher Scientific, Inc.

Techniques: Immunohistochemical staining, Concentration Assay, Clinical Proteomics, Enzyme-linked Immunosorbent Assay